Sign In | Join Free | My polstate.com
polstate.com
Products
Search by Category
Home >

9002-07-7 Sequencing Grade Trypsin , Genetically Engineered Methylation Modified Protein Expressed In E.Coli

Categories Sequencing Grade Trypsin
Brand Name: yaxin
Model Number: SRT0201
Certification: NSF ISO 9001 2015
Place of Origin: SHANGHAI CHINA
MOQ: 100ug
Price: $98
Supply Ability: 1000000000
Delivery Time: 2-3 work days
Packaging Details: 100ug/1mg
SOURCE: Recombinant E. coli
APPEARANCE: White or off white, or yellowish powder
Specific activity: ≥4500 USP units/mg pro.
SDS-PAGE: Single major band
Molecular Weight(SDS-PAGE): 24.0±2.4 kDa
Purity(HPLC): ≥95%
  • Haven't found right suppliers
  • Our buyer assistants can help you find the most suitable, 100% reliable suppliers from China.
  • And this service is free of charge.
  • we have buyer assistants who speak English, French, Spanish......and we are ready to help you anytime!
  • Submit Buying Request
    • Product Details
    • Company Profile

    9002-07-7 Sequencing Grade Trypsin , Genetically Engineered Methylation Modified Protein Expressed In E.Coli

    Sequencing Grade Modified Trypsin is a genetically engineered methylation modified protein expressed in E.coli


    Sequencing Grade Modified Trypsin

    Catalog Number:SRT0201

    CAS:9002-07-7
    EC:3.4.21.4

    Storage temperature: 2-8℃

    Source: Sequencing grade modified trypsin is a genetically engineered methylation modified protein expressed in E.coli

    Protein Sequence:The amino acid sequence of sequencing grade modified trypsin is identical to porcine pancreas-derived trypsin


    1.DESCRIPTION

    YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.


    2.MAIN FEATURES

    SourceRecombinant E. coli
    AppearanceWhite or off white, or yellowish powder
    Specific activity≥4500 USP units/mg pro.
    SDS-PAGESingle major band
    Molecular Weight(SDS-PAGE)24.0±2.4 kDa
    Purity(HPLC)≥95%


    3.RECOMMEND USAGE

    It is recommended to dissolve or dilute with 50 mM HAc or 1 mM HCl. When used, dilute in 50 mM NH4HCO3 or ph7.0-8.0 buffer solution. 1 mM CaCl2 is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.


    4.STABILITY OF STORAGE AND TRANSPORT

    Stability of storage: Sequencing grade trypsin lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃. It is no activity loss after 5 times repeated freezing and thawing.

    Stability of transport: The product is stable by blue ice insulation transport.


    5.PRODUCT ADVANTAGE

    Sequence grade recombinant protein: high specificity,good stability.

    Animal origin free: sequencing grade modified trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.

    Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

    High purity: Higher specific activity.Host protein residues is less than the limits of biological products.

    Lyophilized powder: The product is lyophilized powder and is easy to store and transport.

    Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

    Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.


    6.PRODUCT USAGE

    The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.


    7.RELATED PRODUCTS

    Recombinant enterokinase

    Recombinant V8 protease

    Sequencing grade chymotrypsin

    Sequencing grade carboxypeptidase B

    Quality 9002-07-7 Sequencing Grade Trypsin , Genetically Engineered Methylation Modified Protein Expressed In E.Coli for sale
    Send your message to this supplier
     
    From:
    To: Shanghai Yaxin Biotechnology Co.,Ltd.
    Subject:
    Message:
    Characters Remaining: (0/3000)
     
    Inquiry Cart 0