Edta Trypsin Proteomics Grade Tpck Trypsin Recombinant Trypsin
Enzyme Commission (EC) Number: 220.127.116.11
Molecular Weight: 23.3 kDa (bovine & porcine)
Extinction Coefficient: E1% = 12.9 - 15.4 (280 nm)
pI: 10.1 - 10.5(bovine)
Trypsin is a member of the serine protease family. Trypsin cleaves
peptides on the C-terminal end
of lysine and arginine amino acid residues. The pH optimum of
trypsin is pH 7.0 - 9.0. The enzyme
is inhibited by serine protease inhibitors, e.g. PMSF, aprotinin,
soybean trypsin inhibitor and by
metal chelating agents, e.g., EDTA.
Recombinant Porcine Trypsin is a genetically engineered protein
expressed in E.coli and purified
by high pressure liquid chromatography. There are no any other
contaminating enzyme activities.
No protease inhibitors such as PMSF are contained in the
Animal origin free:The use of recombinant porcine trypsin eliminates the risk of virus
presence, and other potential adventitious agents found in
animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin
belongs to the AOF level 3.
Stablility:A sterile recombinant trypsin lyophilized powder
eliminates the contamination risks and decreases the chance of
activity loss in the process of transport and storage.
1) Recombinant porcine trypsin provides increased specific activity
and eliminates contaminating proteases activities found in
2) No other contaminating proteases such as chymotrypsin or
|Specific activity||≥3800 USP units/mg pro|
|Purity(RP-HPLC)||NLT 70% β-trysin, NMT 20% α-trypsin|
|Contaminant activity||No chymotrypsin, carboxypeptidase A, and other protease
Unit Definition:One USP unit of trypsin activity will produce a
Delta A253 of 0.003 per minute
in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a
substrate (1cm light path).
To prepare 1-10mg/ml recombinant trypsin with 1mM HCl. The ratio to
aimed protein is 1:50 to
1:1000 (w/w), the optimum pH is pH7-10.
Storage And Stability
Recommend recombinant trypsin lyophilized powder stored under 2-8℃
in sealed container. It is stable within 24 months.After dissolved,
it should be stored under -20℃, It is stable within 24 months and
above 90% activity remained after 10 times repeated freezing and