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Recombinant Trypsin Lyophilized Powder Trypsin for Mass Spectrometry

Categories Recombinant Trypsin
Brand Name: YaxinBio
Model Number: RPT0201
Certification: ISO9001
Place of Origin: China
MOQ: 10mg
Price: 65$/10mg
Payment Terms: T/T
Packaging Details: ice packaging
Supply Ability: 1000g per week
Product: Recombinant Porcine Trypsin
Physical form: White lyophilized powder
Specific activity: ≥3800 USP u/mg pro
Purity: > 99%
Related Product: Recombinant Protein A
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Recombinant Trypsin Lyophilized Powder Trypsin for Mass Spectrometry

Recombinant Trypsin Lyophilized Powder Trypsin for Mass Spectrometry


Description


Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end

of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents, e.g.EDTA.

Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified

by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.


Advantages


Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence,

and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant

Porcine Trypsin belongs to the AOF level 3.

Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.


Main Features


Source

E. Coli

Purified by

HPLC

Purity(RP-HPLC)

NLT 70% β-trysin, NMT 20% α-trypsin

Contaminant activity

No chymotrypsin, carboxypeptidase A, and other protease contaminant.


Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute

in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).


Recommend Usage


Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.


Storage Instructions


Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable

within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and

above 90% activity remained after 10 times repeated freezing and thawing.


Email:Zhaozhi@yaxinbio.com

Product Tags:

porcine trypsin

  

cell culture trypsin

  
Quality Recombinant Trypsin Lyophilized Powder Trypsin for Mass Spectrometry for sale
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